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D-Luciferin Firefly, potassium salt
D-Luciferin is a substrate for firefly luciferase (Fluc) used for imaging the expression of the luc marker gene and luciferase-fusion conjugates in living cells, tissues and organisms. ATP is a cofactor for the bioluminescence reaction. Therefore, the luciferase-luciferin is also used to measure the concentra...More info
Tetrazolium Red (TTC) is used to visualise dehydrogenase enzyme activity. Initially the tetrazolium solution is colorless but changes to red when it comes into contact with hydrogen. TTC is used in a biochemical viability test for seeds. The test relies on dehydrogenase enzymes to release hydrogen ions which ...More info
Neutral red is a dye used in histology for staining nuclei red and it is also a pH indicator (transition: pH 6.8 - 8.0 red to yellow). As such it is used in microbial culture media for differentation of bacteria. For example MacConkey agar contains Neutral red and lactose (the sugar in milk). Acidification by...More info
The review covers basic science of Luciferase enzymes, mechanistic aspects of FLuc bioluminescence, S.A.R of Luciferins and top-notch discoveries in the field. Particular attention is given to the highly promising area of in vivo bioluminescence.
In this product minireview we discuss the basic science of Luminol Chemiluminescence, its applications and the main areas of innovation in the field.
S. aureus / MRSA forms red colored colonies on solid growth media with Biosynth's new substrate ALDOL 515 Phosphate (Cat.No A-4721_P00). In contrast to convenitional chromogenic substrates there is no growth inhibition caused by this substrates, this results in significant bgger positive colonies.
Also the read-out is fast: clear-cut signals are seen after only 16 hours of incubation of agar plates containing ALDOL 515 Phosphate.
Detection of beta-galactosidase positive bacteria on solid media
Known chromogenic enzyme substrates such as X-Gal and Magenta-beta-D-Gal form bacteria colonies with colors ranging from blue to red on solid (agar) media. This limitation of the available colors may impede the simultaneous detection of multiple enzymes on one agar plate. Therefore, we developed new substrate colors: Jalapeño green-Gal, Bronze-Gal, Crimson-Gal, and Espresso-Gal. These substrates rapidly develop colored colonies of beta-galactosidase positive bacteria E. coli and coliforms.
RUG™ – novel beta-glucuronidase substrate
Biosynth's novel patented substrate RUG™ (Resorufin-beta-D-glucuronicacid methyl ester, Biosynth Cat. No. R-2155_P00) is transfered by E. coli into the very robust and bright dye Resorufin. Resorufin is one of the most intensely fluorescent materials known today. Hence Resorufin-beta-D-glucuronide represents the perfect agent to detect E. coli bacteria at very low concentrations.
Biosynth viF™ fluorescent dyes and labels cover a range of emission wavelengths from blue to near infra-red (NIR). They are characterized by high optical and chemical stability for long lasting fluorescent signals even under demanding test conditions.
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