Leading Epigenetics Labs Across the World Contribute to Successful Beta Trial of Cambridge Epigenetix’s TrueMethyl Kit

Cambridge Epigenetix Ltd (CEGX), a leader in epigenetics tools innovation, has published the results of a successful beta trial evaluating their pioneering TrueMethyl oxidative bisulfite sequencing (oxBS-Seq) technology.

As a key step in the product validation process, 13 leading epigenetics labs in the US, Europe and Japan were provided with TrueMethyl beta-kits for independent and rigorous testing using a shared panel of control samples. The participants selected represent a broad cross-section of labs leading a revolution in the understanding of epigenetic science and included

Professor Andy Feinberg — The Johns Hopkins University
Dr George Vassiliou — The Wellcome Trust Sanger Institute
Dr Nessa Carey  — Neusentis-Pfizer Pharmaceutical Research Unit
Professor Adrian Bird — The University of Edinburgh
Professor Stephan Beck — University College London
Professor Jonathan Mill  — University of Exeter & King's College London
Professor Jörn Walter — Universität des Saarlandes
Professor Wolf Reik and Dr Miguel Branco — The Babraham Institute
Professor Hiro Sasaki  — Kyushu University
Dr Dirk Schübeler — Friedrich Miescher Institute for Biomedical Research
Dr Skirmantas Kriaucionis — University of Oxford Ludwig Institute for Cancer Research
Dr Marian Mellén — The Rockefeller University

The successful beta trial results demonstrate that TrueMethyl’s innovative science and robust workflow deliver reliable and consistently high performance. Dr George Vassiliou and Dr Malgorzata Gozdecka of the Wellcome Trust Sanger Institute said: “We found the TrueMethyl kit a technically simple and rapid method for parallel genome-wide detection of cytosine methylation and hydroxy-methylation. Most importantly, the user-friendly kit enabled the generation of reliable and highly reproducible data sets.”

"The beta trial's success proves the accuracy and reproducibility across multiple labs of oxBS-Seq and was the final step in our rigorous development of commercial grade TrueMethyl kits” commented Dr Tony Smith, CTO of Cambridge Epigenetix. “We are delighted at the level of demand at launch and expect TrueMethyl to become the industry standard."

CEGX’s application note (available for download at www.cambridge-epigenetix.com) describes the design of the international beta trial and summarizes the key results, illustrating the outstanding performance of TrueMethyl for quantitative detection and discrimination of cytosine, 5mC and 5hmC at single-base resolution.