Preparing samples of cannabis plant for THC/CBD analysis15 Jan 2019
Porvair Sciences has created a solid, stable and strong microplate that can withstand the applied forces in bead-beaters while maintaining a standard ANSI/SLAS footprint and key dimensions.
Porvair Sciences has reported on a highly effective protocol, using its Plant Genomics microplate, for preparing cannabis samples for analysis of Tetrahydrocannabinol (THC) and Cannabidiol (CBD).
With increasing legalization of cannabis for medicinal and research purposes, demand for accurate and high throughput analysis of its active ingredients has dramatically risen. Cannabis plant material contains several cannabinoids for which the human brain has evolved receptors. The two most important are the psychoactive THC and the analgesic CBD which has therapeutic uses. The ratio of THC and CBD is crucial for differentiating medicinal versus recreational cannabis. This ratio is important for taxation authorities, medical regulators and growers alike.
Accurate determination of the THC/CBD ratio is critically determined by effective sample preparation of the samples of cannabis leaf, bud and flower. Traditional sample preparation methods have used bead-beating or grinding mills to homogenise these materials. When conducted in polypropylene microplates the extreme forces applied by these machines often cause damage to the plates leading to cracking, leaking and ultimately, cross-contamination.
Porvair Sciences has applied modern design concepts and mould analysis to this problem to create a solid, stable and strong microplate that can withstand the applied forces in bead-beaters while maintaining a standard ANSI/SLAS footprint and key dimensions. By adding reinforced walls to the bottom of the well and backing these with supporting ribs on the underside of the plate, Porvair Sciences has produced a 2 mL square well block (Plant Genomics plate) that can be used safely and with confidence for cannabis plant extraction. The Plant Genomics plate has a generous working volume of 1.90 mL per well in a 45-mm high plate. The polypropylene used to mould the plate is pure, virgin, extractable free polymer, rigorously tested for leachates thereby ensuring no contamination from the plate will affect THC/CBD analysis.
In the described protocol, sample preparation using the Plant Genomics plate in a GenoGrinder takes just 2 minutes. Following this, the dry ground cannabis powder is transferred into the wells of a Porvair P3 filter microplate for extraction under agitation with methanol or isopropanol to dissolve the oily residues in the plant material. After agitation, the P3 plate is placed onto a Porvair Microlute manifold and vacuum applied to draw the purified extract down through the filter and into the wells of a collection plate. The collection plate containing the organic extract is then taken for HPLC analysis to determine the THC/CBD ratio. To determine total THC and CBD in the Cannabis plant sample, the purified extract should be gently evaporated to dryness using a Porvair MiniVap blowdown evaporator. The sample should then be redissolved in a known volume of solvent ready for HPLC analysis. Both methodologies are reported to be straightforward and repeatable.
New API screening program strengthens Particle Sciences' nanomilling offering
22 Jan 2019
Advanced formulation techniques such as nanomilling may provide an excellent route to improved bioavailability and enhanced therapeutic effect.Read more
Allergan to establish R&D presence in Cambridge, Massachusetts
10 Jan 2019
The new, strategic presence will allow the company to more easily interact and engage with venture firms and start ups in the area.Read more
Expedeon signs supply and license agreement for is Lightning-Link technology
9 Jan 2019
The technology will enable Cell Guidance Systems to manufacture TRIFic detection assays on-demand with highly reproducible and scalable results.Read more
WuXi AppTec expands site in California for pharmaceutical R&D services
13 Dec 2018
Expanded facility will establish a center of excellence in screening, discovery biology, pharmacology, and small molecule process R&D including Phase I GMP manufacturing.Read more
Cambrex expands analytical capabilities
12 Dec 2018
The $1 million investment will assist in getting new products to commercial launch faster.Read more
MIT engineers repurpose wasp venom as an antibiotic drug
9 Dec 2018
Altered peptides from a South American wasp’s venom can kill bacteria but are nontoxic to human cells.Read more
Spanish CDMO working ahead of EU serialisation deadline
6 Dec 2018
Company now able to serialise commercial batches manufactured by them for clients; it can also offer standalone serialisation services as an outsourcing partner.Read more
Fluidic Analytics raises $31m to launch new lab tools and unlock biology behind major diseases
5 Dec 2018
The financing will power the global commercial launch of the company's Fluidity One system.Read more
OnDosis and Recipharm partner to advance a novel treatment regimen for ADHD
4 Dec 2018
The new treatment will leverage OnDosis’ proprietary technology, which integrates oral drugs in form of micro units and individualised dosing, in a handheld device.Read more
NIH to evaluate effectiveness of male contraceptive skin gel
28 Nov 2018
The gel formulation includes the progestin compound segesterone acetate, in combination with testosterone, and is applied to the back and shoulders and absorbed through the skin.Read more
Are you a supplier
Here's what we can do for you
- Generate quality leads for your business
- Stay visible for 365 days of the year
- Receive product inquiries and respond to meeting requests directly
- Improve company online presence through Search Engine Optimisation