Custom Phospho-Specific Antibody Production Service

Drawing upon many years’ experience, AMSBIO has developed a custom phospho-specific antibody production service that is both reliable and produces highly specific quality antisera.

Phospho-specific antibodies are widely accepted for use in defining regulatory mechanisms that control cell functions such as activation of enzymes and receptors, cellular transcription, signal transduction, and cell signalling networks. Phospho-specific antibodies from AMSBIO are affinity-purified rabbit polyclonal or monoclonal antibodies that are monospecific for a target protein that is phosphorylated on a specific tyrosine, threonine or serine residue.

AMSBIO’s custom phospho-specific polyclonal antibody service includes synthesis of phosphorylated and non-phosphorylated peptides, conjugation to a carrier protein, immunization and anti-sera production. All antibodies are then purified using a two-step immuno-affinity method. The purified phospho-specific antibody is fully characterised using both dot-blot and ELISA techniques to ensure product of the highest consistency and quality. Hundreds of custom phospho specific antibodies have been successfully made by AMSBIO and supplied to leading international research groups [1,2].

References

1. C.J. Cox, et al., 'Brain Human Monoclonal Autoantibody from Sydenham Chorea Targets Dopaminergic Neurons in Transgenic Mice and Signals Dopamine D2 Receptor: Implications in Human Disease,' The Journal of Immunology (2013).

2. K.M. Brown, et al., 'Cyclic AMP-Specific Phosphodiesterase, PDE8A1, is Activated by Protein Kinase A-Mediated Phosphorylation,' Febs Letters, 586(11), 1631–1637, Elsevier (2012).