Target ion channels and pore-forming proteins are reconstituted into a defined artificial membrane and measured directly at the single-channel level. This approach enables target-focused functional analysis while minimizing interference from endogenous ion channels and complex cellular backgrounds.
Our measurement platform was co-developed with Toray Engineering Co., Ltd. and integrates a 16-channel planar lipid bilayer chip, picoamp current measurement system, and automated waveform analysis software. The system enables ion channel reconstitution, electrophysiological recording, and functional data analysis in an integrated workflow.
We support compound profiling, pharmacological characterization, candidate selection, selectivity evaluation, and lead optimization decisions. Electrophysiological readouts can include single-channel current, conductance, open probability, voltage dependence, activation or inhibition, concentration-dependent compound effects, and IC50.
A particular strength of our platform is access to organelle ion channels, including mitochondrial, endoplasmic reticulum (ER), and lysosomal membrane channels that can be difficult to evaluate using conventional cell-based electrophysiological assays. As an example, we have successfully reconstituted and functionally measured human VDAC1, VDAC2, and VDAC3 (voltage-dependent anion channels) from the mitochondrial outer membrane.
Our contract research services cover feasibility assessment, assay development, measurement condition optimization, electrophysiological measurement, data analysis, interpretation, and reporting.
Maqsys supports drug discovery researchers seeking direct functional evidence of compound–ion channel interactions, including challenging and emerging ion channel targets.
