PyroSup® Monocyte Activation Test (MAT) Kit

Monocytes, as key components of the human innate immune system, recognize pyrogens through Toll-like receptors (TLRs) and respond by releasing proinflammatory cytokines, such as interleukin-6 (IL-6). The concentration of IL-6 secreted into the culture supernatant reflects the degree of pyrogenic stimulation.
The PyroSup MAT Kit utilizes cryopreserved human monocytic cells (MAT cells) and a human IL-6 ELISA assay to quantitatively determine the presence of pyrogens in test samples. This method enables detection of both endotoxin and non-endotoxin pyrogens (NEPs), providing a physiologically relevant alternative to traditional rabbit pyrogen test (PRT).