Integrated DNA Technologi?es Enhances its qPCR Probe Portfolio

Integrated DNA Technologies (IDT), the world leader in oligonucleotide synthesis, has further expanded its portfolio of PrimeTime® qPCR products with the launch of two new probes. The PrimeTime Eco Probe and PrimeTime Mini LNA Probe complete the existing offering, providing new options for customers performing gene expression and genotyping experiments.

Developed to support customers looking for a cost-effective option at the mid-scale range, the Eco Probe is delivered at a normalised yield of 2.5 nmoles, sufficient for approximately 500 reactions, bridging the space between the existing PrimeTime Mini qPCR Probe (0.5 nmole normalized yield, 100 reactions) and the 100 nmole synthesis scale (minimum guaranteed yield of 10 nmoles). If ordered with the option of an internal ZEN quencher, the double-quenched PrimeTime Eco Probe will generate less background noise, while increasing end point signal, to significantly boost qPCR sensitivity and precision when compared to traditional single-quenched probes. The Eco Probe is available with a FAM/ZEN/IA Black FQ dye/quencher combination.

When genotyping via PCR, it is often useful to be able to perform a few reactions using a small subset of probes, in order to test and consequently optimise LNA probe design. The new PrimeTime Mini LNA Probes enable customers to do as few as 100 reactions, allowing preliminary experiments to be performed at a low initial cost.

Guidelines for LNA probe design are available online, under the Overview tab to aid users in their initial design. IDT can also provide full design support, if required — design fees may apply. The Mini LNA Probes join the existing 250 nmole PrimeTime LNA Probes, which provide 8000 reactions and are ideal for large-scale screening.