Routine Generation of Powder Samples from HPLC Fractions

Genevac reports that its HT Series II Evaporator using the Fast Lyophilisation, or LyoSpeed process is enabling a growing number of pharmaceutical labs to routinely dry HPLC fraction samples to a powder form, such that the samples can then be accurately weighed, easily sub-sampled and redissolved.

When working with certain HPLC solvents, such as water and acetonitrile, a fully dried result can be hard to achieve due to interactions with the dissolved sample — esulting in the formation of a gum or oil.

To provide a rapid, high-throughput alternative to freeze drying HPLC purification fractions — Genevac developed its proprietary fast lyophilisation (LyoSpeed) methodology for use on the HT Series II Evaporators. Although LyoSpeed works well for most hydrophilic samples, difficulties may arise where the sample cannot dissolve in only water. In such situations, when the organic solvent is removed, the sample crashes out forming an oil which until now required further processing to achieve the desired dry powder form. For such samples and to improve LyoSpeed results generally — Genevac developed the SuperCool 75 which chills to -75 ºC and operates at higher power than any other commercially available condenser.

Customer trials have shown that the Genevac HT Series II Evaporator/SuperCool 75 combination produces powdered lyophilisation samples using the LyoSpeed methodology from even the most difficult to handle samples with a success rate as high as 95%

For further information please visit www.genevac.com/en/ProductDetail1.asp?S=3&V=1&G=5&Product=12 or contact Genevac now on +44 1473 240 000/+1 845 255 5000 or email [email protected]