Trypsin

Trypsin
Product Description

Trypsin is a serine endoprotease. It specifically hydrolyses peptide bonds at the C‑terminal side of lysine and arginine residues (except when followed by proline).

Native trypsin is synthesized as inactive trypsinogen in animal pancreas. For modern biopharma production, recombinant animal‑free trypsin is widely adopted to eliminate risks of animal‑derived virus / TSE contamination, with consistent batch‑to‑batch performance.

  • Molecular weight: ~23‑24 kDa
  • Optimal pH: 7.0‑9.0, maximum activity around pH 7.5‑8.5
  • Optimal temperature: 30‑37 °C
  • Inhibition: Inhibited by serine protease inhibitors (PMSF, TLCK) and metal chelators such as EDTA; Ca²⁺ improves enzyme stability and reduces autodegradation.

Yifan Pharmaceutical Co., Ltd

  • Country/Region:
    China
  • On CPHI since:
    2024
  • Certificates:
    4
  • Employees:
    5000+
Company types
Biopharmaceutical company
CMO/CDMO
Distributor/Import Export
Generics/Biosimilars Manufacturer
Licensee
Manufacturer/Innovator
Pharmaceutical company
Primary activities
Biopharmaceutical
Pharmaceutical Company (innovator finished products)
Specifications
  • CAS Registry Number
    9002‑07‑7
  • Details
    Key Applications
  • Biopharmaceutical manufacturing (core usage) Critical processing enzyme for insulin and insulin‑analog biosimilar production. Together with Carboxypeptidase‑B (CPB), trypsin cleaves pro‑insulin to produce mature active insulin API.
  • Cell processing: Cell detachment for vaccine, cell‑therapy workflows.
  • R&D: Protein sequencing, mass‑spectrometry sample preparation, recombinant‑protein cleavage.

  • GMP‑grade recombinant trypsin is standard raw material for macromolecule drug manufacturing.

  • Selling Points
    GMP Manufacturing
  • Model
    enzyme
  • Measured In
    gram
  • Yifan Pharmaceutical Co., Ltd

    • Country/Region:
      China
    • On CPHI since:
      2024
    • Certificates:
      4
    • Employees:
      5000+
    Company types
    Biopharmaceutical company
    CMO/CDMO
    Distributor/Import Export
    Generics/Biosimilars Manufacturer
    Licensee
    Manufacturer/Innovator
    Pharmaceutical company
    Primary activities
    Biopharmaceutical
    Pharmaceutical Company (innovator finished products)

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